Overview

Teaching: 0 min
Exercises: 0 min

Questions

• How does NGS sequencing work?

Objectives

• Understand the process of next generation sequencing methods

• Be able to describe sequencing by synthesis methodology

• Practice a mini sequencing project utilizing legos/bricks

DNA Sequencing Lessons

We will utilize tutorials created by Illumina to understand various aspects of the DNA sequencing process. You are required to watch and take notes on the first 3 videos in your OneNote notebook.

Lesson 1: Click this link for a course on Sequencing Fundamentals

• Parts 1, 2, and 3 may be review for you from previous coursework.

Lesson 2: Understanding the NGS Workflow

• An overview of Next Generation Sequencing for Beginners.

Lesson 3: Click this link for an overview or Illumina sequencing by sythesis

• An overview of sequencing by synthesis. This is the methodology used to obtain the .fastq files you will be working with.

Lesson 4 - Optional: Click this link for a lesson on an Introduction to Sequencing

Lesson 5 - Optional: Click this link for a video on an Introduction to Key Concepts of Sequencing Data Analysis

Image of Paired-End Reads

Figure 1: Image from Illumina demonstrating Paired-End Reads. Each fragment will be sequenced twice resulting in Read 1 and Read 2 for every fragment. Therefore, there will be two .fastq files for every sample sequenced. One file will contain all of the reads for Read 1 and the other file will contain all of the reads for Read 2.

Lego Activity

Complete the following Lego Activity (with or without Legos) and upload to your OneNote Notebook.

Link to the NGS Lego Activity

Link to file using PPT “bricks” to manipulate virtually if you don’t have legos to work with

Link to reference genomes for Lego Activity

These files can also be found in the content library of your OneNote notebook.

Key Points

• These lessons help you understand NGS sequencing methods and how .fastq files are generated